技术研究TECHNICAL
  • Biotinylated Recombinant Proteins
    Biotinylated Recombinant Proteins
    2021-06-14
    Gaussia princeps Luciferase Biotinylated Avitag fusion (Cterminus) Gaussia princeps luciferase is a bioluminescent protein. It is ATP-independent, requiring only coelenterazine and O2 as substrates. This purified protein is fused with Avita...
  • Vectors
    Vectors
    2021-06-14
    Avidty teamed with Atum (formerly DNA 2.0) to offer our customers an easy solution to AviTagging their E. coli expressed proteins. The new vectors employ the Atum ElectraTm system for easy cloning from a PCR product. To minimize steric conf...
  • In vivo Biotinylation-Strains
    In vivo Biotinylation-Strains
    2021-06-14
    STRAIN AVB101 Strain AVB101, an E. coli B strain (hsdR, lon11, sulA1), contains pBirAcm , an IPTG inducible plasmid containing the BirA gene engineered into pACYC184. It is compatible with most cloning vectors and is maintained with chloram...
  • In vitro Biotinylation of Avitag with Enzyme
    In vitro Biotinylation of Avitag with Enzyme
    2021-06-14
    BIOTIN PROTIEN LIGASE Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5 adenylate and transfers the biotin to biotin-accepting proteins. It also functions as a biotin operon repressor. This protein is encoded by the bi...
  • Monoclonal antibody against C-terminus presented AviTag
    Monoclonal antibody against C-terminus presented AviTag
    2021-06-14
    A monoclonal antibody was developed that reacts only to the AviTag when fused to the C-terminus of desired protein. It will identify C-terminus fusions on western blots and has been immobilized on agarose beads for purifications purposes. T...

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